Journal: Frontiers in Immunology

Article Title: Toll-Like Receptor 7 Enhances Rabies Virus-Induced Humoral Immunity by Facilitating the Formation of Germinal Centers

doi: 10.3389/fimmu.2019.00429

Figure Lengend Snippet: TLR7 is indispensable for optimal antibody production after RABV immunization. TLR7 −/− ( n = 12), TLR3 −/− ( n = 10), or WT mice ( n = 11–12) were immunized i.m. with 10 6 FFU RABV vaccine strain LBNSE in the hind legs. At indicated times post immunization, sera were collected for measuring RABV-specific total IgG (A) and VNA titers (B) . Error bars represent standard error of mean (SEM) ( * P < 0.05; ** P < 0.01; *** P < 0.001).

Article Snippet: Bound Ab was detected using HRP-conjugated Abs (IgG (1:1,000), IgG1 (1:1,500), IgG2a (1:1,500), IgG2b (1:2,000), IgG3 (1:1,500), or IgM (1:2,000) (Boster, Wuhan, China).

Techniques:

Journal: Frontiers in Immunology

Article Title: Toll-Like Receptor 7 Enhances Rabies Virus-Induced Humoral Immunity by Facilitating the Formation of Germinal Centers

doi: 10.3389/fimmu.2019.00429

Figure Lengend Snippet: TLR7 facilitates the formation of GCs. (A,B) TLR7 −/− and WT mice were immunized i.m. with 10 6 FFU LBNSE in the hind legs. Draining LNs were excised, and tissue sections were prepared and stained for GCs (GL-7, red; B220, blue; and IgG, green). Scale bars represent 200 μm. Representative sections are shown in (A) Numbers of GCs (GL-7 positive cell clusters labeled with white arrows) in the draining LNs are calculated and shown in (B) ( n = 3). (C) Blood samples were collected at indicated time points and the concentration of serum CXCL13 was determined by using a commercial ELISA kit ( n = 9). Error bars represent SEM ( * P < 0.05; *** P < 0.001).

Article Snippet: Bound Ab was detected using HRP-conjugated Abs (IgG (1:1,000), IgG1 (1:1,500), IgG2a (1:1,500), IgG2b (1:2,000), IgG3 (1:1,500), or IgM (1:2,000) (Boster, Wuhan, China).

Techniques: Staining, Labeling, Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Immunology

Article Title: Toll-Like Receptor 7 Enhances Rabies Virus-Induced Humoral Immunity by Facilitating the Formation of Germinal Centers

doi: 10.3389/fimmu.2019.00429

Figure Lengend Snippet: TLR7 facilitates Th1-biased immune responses after RABV vaccination. TLR7 −/− and WT mice were immunized i.m. with 10 6 FFU LBNSE in the hind legs. Spleens were collected at 7 d.p.i. and subjected to ELISpot assay to measure RABV-specific IFN-γ (A,B) and IL-4 (C,D) producing cells ( n = 3 mice/ group). (E–G) Blood samples were collected at the indicated times p.i. for analysis of RABV G-specific IgG1 (E) , IgG2a (F) , and IgG2b (G) ( n = 10). Error bars represent SEM. ND, not detectable. NS, no significantly difference ( * P < 0.05; *** P < 0.001).

Article Snippet: Bound Ab was detected using HRP-conjugated Abs (IgG (1:1,000), IgG1 (1:1,500), IgG2a (1:1,500), IgG2b (1:2,000), IgG3 (1:1,500), or IgM (1:2,000) (Boster, Wuhan, China).

Techniques: Enzyme-linked Immunospot